Taken from the Official Forums
Event-related
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Heroines' Holdfast is a special battlefield created to celebrate FINAL FANTASY XI's tenth anniversary and takes place on Nyzul Isle. The holdfast houses multiple heroines; one for each floor. Participants make their way through certain floors and finally battle the heroine who awaits them in her chamber on the corresponding floor.
Entry Requirements
Have one's main job at level 99.
Form a party of three to six people.
There is a time restriction of fifteen minutes, which can be extended by an additional fifteen minutes each time a heroine is defeated.
Heroines' Holdfast Overview
Trade an Athena Orb to the Runic Seal at the Nyzul Isle staging point (J-9) in the : Alzadaal Undersea Ruins to enter the battlefield. You can obtain an Athena Orb through the FFXI Forum contests and Golden Gobbie Bag Drawings.
*The Athena Orb will disappear upon entering the holdfast.
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Transfer to the antechamber.
There are five runes of transfer in the antechamber that teleport participants to each heroine's floor, and deciding which heroine your party shall do battle with before trading your Athena Orb should save valuable time at this juncture.
The following runes of transfer teleport players to the corresponding heroine's floor:
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* : Starting Location
Rune of Transfer #1:
Lion
(Can't wait to see my favorite Zilart/Original Game NPC again!)
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(Favorite all time NPC for many. Main character of the CoP Storyline.)
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(Some may know her by other names, but here we fight ToAU's court puppeteer and her two puppets.)
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(From the Maws to your face, WoTG's dancer has arrived)
Rune of Transfer #5:
Mumor
(Shimmering Shining Samba!!!... or something?)
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The acquisition of other rewards will no longer be interrupted when a rare item already in one's possession is among the spoils obtained from a Riftworn Pyxis after selecting "obtain all."
Enemies' resistance to enfeebling magic has been adjusted.
It is now possible to reenter a battlefield after being ejected due to all participants having been KO'd.
In addition, the distance that enemies roam has been adjusted. (Hopefully the HNMs will stop having tea-parties long enough to be pulled. >.>)
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When moving to the next floor during Nyzul Isle Investigation and Nyzul Isle Uncharted Region, characters will now zone in at different coordinates around the Rune of Transfer. (Yay, I no longer have to wait for all the Elvaans and Humes I play with to get the hell out of the way so I can move!.. on another note... Where do they teleport Galkas? I don't see one here.)
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Battle-related
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The value for each point has been modified from DEF+2 to DEF+5 (Nice!)
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The value for each point has been modified from DEF+1 to DEF+2.
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A log message now appears when a monster resists an enfeebling spell which enables the player to determine whether a monster possesses complete resistance to a particular type of enfeebling magic. (Very awesome!)
As long as the monster does not completely resist the effect, it is possible to inflict enfeebling magic upon them. The number of affectable monsters has drastically increased. (If it can be paralyzed, Yopo and Cowbox can paralyze it!)
A new system has been added in which using enfeebling magic multiple times will lower the target enemy's resistance. This system is known as "Immunobreak." (I really like this idea.)
When an Immunobreak occurs after the enemy has resisted a particular enfeebling magic, the resistance of the enemy to that effect will decrease by a certain amount.
Immunobreaks can stack and continually lower an enemy's resistance.
The more times the enfeebling magic is resisted, the greater the likelihood that an Immunobreak will occur.
When the enfeebling effect is successfully applied, the resistance will reset. (Balance!)
If the effect is continuously applied, the enemy will slowly gain more resistance, and eventually Immunobreaks will no longer occur.
Note that some monsters have complete resistance to enfeebling effects.
(Immunobreaks will not occur on enemies which have complete resistance.)
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Protect / Protectra / Protect II / Protectra II / Protect III / Protectra III / Protect IV / Protectra IV / Protect V / Protectra V / Knight's Minne / Knight's Minne II / Knight's Minne III / Knight's Minne IV / Knight's Minne V
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Foe Requiem / Foe Requiem II / Foe Requiem III / Foe Requiem IV / Foe Requiem V / Foe Requiem VI / Foe Requiem VII
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Item-related
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(Hell yeah, the All-In-One Staff. 8 Open spots here I come!.. pending on if it really is better)
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Cordial Invite / Mercenary Camp Entry Slip / Martial Ball Invite / Training Grounds Key / Pest Repellant
System-related
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[Windows and Xbox 360 Only]
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(I personally really like these icons. After 10 years, it's nice to see some changes!)
Various issues have been resolved as well.